Our company launched the "You Ask Me Answer" activity of fetal bovine serum

A teacher Xie from Ningbo, Zhejiang asked: "How can I prevent the onset of serum deposits?"

The technical specialist replied: When freezing the serum, please follow the gradual freezing method (-20 ° C to 4 ° C to room temperature). If the temperature changed when the serum is frozen is too large, the test will show very simple seizure. When frozen together, please shake it at any time to make the temperature and composition uniform and reduce the occurrence of deposition. If it is necessary to heat inactivate the serum, please follow the guidelines of 56 ° C for 30 minutes, and shake it at any time. If the temperature is too high, the time is too long or the shaking is uneven, it will cause an increase in sediment.

Then Teacher Xie asked, "I have been rumored to be able to avoid heat inactivation. I would like to take this opportunity to ask, by the way, is it necessary to do heat inactivation?"

The technical specialist replied: The experiments showed that properly processed heat-inactivated serum is not needed for most cells. The treated serum only slightly promoted the growth of the cells, or had no effect at all, and even the high temperature treatment generally affected the quality of the serum, which caused the decline of the cell growth rate. The composition of sediments after heat-treated serum will increase significantly. Investigation of these sediments under an inverted microscope, such as “small black spots”, often makes researchers mistakenly think that the serum is contaminated, and put the serum in 37 In the environment of ℃, this sediment will increase more, which makes the researchers mistakenly think that it is the cleavage and expansion of microorganisms. We therefore advocate that you can eliminate the need for heat treatment if not necessary.

Another Yi teacher from Lijiang City, Yunnan Province asked, "It's hard for me to freeze the serum. How can I freeze the product so as not to affect the quality?

The technical specialist replied: We recommend that you take the serum out of the freezer and melt it in a refrigerator at 2-8 ° C, and then melt it at room temperature. However, it is necessary to note that during the melting process, it is necessary to shake regularly.

Next, Teacher Ma of Anshan City, Liaoning Province asked, "How is 500ml serum generally divided? How to store it?"

The technical specialist replied: If the serum is stored, it should be done at -5 ° C to -20 ° C. If it is stored at 4 ℃, please do not exceed one month. If you are unable to use one bottle at a time, we recommend that you aliquot the serum into a suitable sterilized container and put it back in the freezer. A 500ml serum should be dispensed as follows:

Pre-sterilized serum bottle

2. Frozen slowly, and then leave it at room temperature to equilibrate.

3. Inactivation: water bath, 56 ℃, 30 minutes, and shake evenly at any time. Remove and immediately cool on ice to room temperature. During heat inactivation, regular shaking can reduce the onset of deposition.

4. Transfer the asepsis into a sterile room, and place the serum into a 50-100ml serum bottle in a clean bench (note that the serum should be quietly shaken for several weeks before mixing, and the serum should be blown out with a pipette. Note: Do not blow out the airtight seal, and store it at -20 ℃ for future use.