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In addition to the quality and quality of the seeds, germination of plant seeds has a lot to do with the external environment such as light, temperature, humidity, and nutrients. The science uses the artificial climate box to monitor and observe the germination process of the seeds in order to understand the influence of the external environment on the germination of the seeds.
Different seed germination requires different dark treatments. Some seeds must undergo dark culture before they germinate. Dark treatments can effectively increase the germination rate of their seeds. For example, dark culture is a necessary condition for the germination of some Paphiopedilum plants. Dark cultivation of seeds facilitates the increase of seed germination rate and the early growth of seedlings.
The aseptic sowing of plant seeds can greatly increase the germination rate, and can obtain a large number of young plants in a short period of time, and the tissue culture seedlings are even and robust, and the survival rate of transplanting is high. This is an economically effective rapid propagation method at the current stage, and is also an industrialized seedling raising. One of the important ways. The seeds can be germinated when the seeds of the plant are aseptically seeded, and the seed germination rate is increased when the seed coat is smashed, the sodium hydroxide is pretreated, or the plant hormone 6-benzylamino adenine (6-BA) is added to the medium.
There are few studies on the effect of dark cultivation time on the seed germination rate during the process of seed germination. In this experiment, under sterile conditions, the effects of chemical reagent pretreatment and dark culture time on the germination of wild Chunlan seeds were studied, and the factors influencing the initial germination time and germination rate of Chunlan seeds were investigated. The seeds were observed and recorded during the germination process. Morphological changes.
The results of Habenaria dentata showed that the seed germination rate was up to 82.0% when cultured in the dark for 20 days and cultured under light. The germination of some plant seeds does not necessarily require dark culture. For example, Lindane et al. showed that the light and dark cultures of the seeds of Renantheraimschootiana were not significantly affected by seed germination when they were sown at aseptic seeding, but the seeds were germinated after 3 weeks of dark culture. Late development is unfavorable. The results of this experiment indicate that light culture after dark culture is essential for the initiation of seed germination. Although dark culture requires the shortest time for germination to start, the final germination rate is not high. However, after a longer period of dark culture, the light was cultured. Although the germination started slowly, the germination rate was higher.
Therefore, suitable conditions for the germination of Chunlan seeds were light cultivation after dark cultivation for 150 days. The different dark processing time required for different seed germination is mainly due to the red light-absorbing phytochrome (Pr) and the far-red light-absorbing phytochrome (Pfr) in the seeds. The seed germination or dormancy depends on the seeds during germination. The established Pfr content and Pfr/(Pr+Pfr) ratio. Chlorophytum seeds germinate in darker cultures that require a longer period of time and require light culture. This may be due to the lower level of Pfr required for the germination of celosia sinensis seeds, thus requiring a longer period of dark culture, whereas light culture favors seed development and root formation. Stem formation.
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