Spore traps study the spore dispersal of white rot in grapes

In the northern grape-growing areas, the average loss rate of white rot in grapes is 15% to 20%, and the fruit loss rate in disease-end years is more than 60%. Studies on the occurrence and prevention of white rot in grapes have been reported both at home and abroad, but there are few studies on the epidemic dynamics of the disease. In this study, daily and hourly spore capture was used as a means to combine the continuous recording of meteorological data to explore the dynamics of conidia scattering and the major meteorological factors affecting the dispersion of white rot pathogens in winery vineyards in the growing season in order to study the disease. The prevalence and predictions provide a theoretical basis. Spore traps are an advantageous tool for capturing spores and support research.

The spore catcher is placed in an empty place in the winemaking vineyard. Every year from June to September, the “He Nong type” electric spore catcher is used to capture the spores on an hour-by-day basis, and then the tape with spores is taken back into the room for milking. The phenol oil was used as a floater, and it was adhered to a clean glass slide and the spore volume was counted under a microscope.

During the entire investigation period, vineyards in the winemaking industry had spores of white rot pathogens, and the number of scattered particles per day varied. In general, the number of conidia in the pre-growth season is greater than in the later period. The survey results showed that there was a significant negative correlation between spore scattering and temperature during each hour of the day and a significant positive correlation with relative humidity. White rot pathogens sporulate at room temperature or slightly above room temperature and humidity above 73%. During the growing season, high daytime temperatures and low humidity are not conducive to sporulation, so spores are scattered in small quantities during the day and in the opposite direction at night. Spores are scattered in large quantities.

The study of the capture of spores by the spore catcher found that when rainfall occurs, the relative humidity of the air is relatively high and the temperature is low, which is favorable for the production of spores. The increase of air flow during a period of time before rain and the impact and splash of raindrops are conducive to spore transmission. Rainfall has created suitable conditions for bacterial infection. Therefore, this mode of transmission is more important for the re-infestation of diseases in the growing season and is more favorable to the disease epidemic. In this experiment, the relationship between conidial spore scattering of white rot pathogen and temperature and humidity was studied. The relationship between conidial spore scattering of white rot pathogen and rainfall, and the role of wind (including wind and wind direction) in it need to be further studied. .

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