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The content of protein in milk is very rich, and the protein therein is a nitrogen-containing organic compound. For the determination of protein content, the sample needs to be heated and digested together with sulfuric acid and a catalyst to decompose the protein, and the decomposed ammonia is combined with sulfuric acid to generate sulfuric acid. Ammonium, then alkalized distillation to ammonia free, absorbed with boric acid and then titrated with hydrochloric acid standard solution, according to the consumption of acid to calculate the nitrogen content, and then multiplied by the conversion factor to obtain the protein content. The use of digester is essential in this process.
Digestion furnace applications, the digestive tubes were placed in each hole in the digestive rack, and then placed in the digester, and immediately open the suction pump valve, so that the suction pump is in the suction state. When the power is turned on, during the initial stage of heating, attention must be paid to observation to prevent the sample from splashing due to abrupt boiling (the initial voltage is adjusted to 180-190V). After the foam is stopped, the voltage is adjusted to 220v, and after cooling for 100-105 min.
When using the semi-micro Kjeldahl method to determine the protein content of milk, a porous well-type electric heating furnace is used, and a suitable inexpensive catalyst is placed before digestion to digest the milk sample, which greatly improves the thermal effect of the digestion sample. At the same time, the digestion time has also been shortened a lot. Milk protein contains 7.1% (mass fraction) of lysine and 2.6% (mass fraction) of histidine. Nitrogen in these two amino acids is not digested completely in a short time, which often leads to low total nitrogen. Digestion furnace digestion time is too long will cause loss of ammonia. The method is used to determine the protein in milk, and the time for digesting one sample is 100-105 min, and the coefficient of variation is 0.62%. The results obtained by this method are accurate, accurate, stable, simple, fast and safe, and can meet the detection requirements of batch samples.
Digestion furnace applications, the digestive tubes were placed in each hole in the digestive rack, and then placed in the digester, and immediately open the suction pump valve, so that the suction pump is in the suction state. When the power is turned on, during the initial stage of heating, attention must be paid to observation to prevent the sample from splashing due to abrupt boiling (the initial voltage is adjusted to 180-190V). After the foam is stopped, the voltage is adjusted to 220v, and after cooling for 100-105 min.
When using the semi-micro Kjeldahl method to determine the protein content of milk, a porous well-type electric heating furnace is used, and a suitable inexpensive catalyst is placed before digestion to digest the milk sample, which greatly improves the thermal effect of the digestion sample. At the same time, the digestion time has also been shortened a lot. Milk protein contains 7.1% (mass fraction) of lysine and 2.6% (mass fraction) of histidine. Nitrogen in these two amino acids is not digested completely in a short time, which often leads to low total nitrogen. Digestion furnace digestion time is too long will cause loss of ammonia. The method is used to determine the protein in milk, and the time for digesting one sample is 100-105 min, and the coefficient of variation is 0.62%. The results obtained by this method are accurate, accurate, stable, simple, fast and safe, and can meet the detection requirements of batch samples.
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